The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. Supported Windows Operating System: See release notes. to View Figures. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Italic and boldface type are used for emphasis. Actual shake speed is based on the microplate format. Prime function 20 amol. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Figure 1. Unparalleled performance on a personalized platform. Ease of use. HTRF IP standard curve. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Bioz Stars score: 98/100, based on 1 PubMed citations. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 9 x 10-15. Figure 4. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). 312 gain calibration failed. StakMax Handling Stacker Base Unit. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. to View Figures. It allows you to explore cellular . ATP (“flash” luminescence using . The M5 can read volumes down to 2uL with the uMax Low. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. 1. ISM Equipment Classification (Group 1, Class A) This equipment is. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. 2 or higher,. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Figure 4. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Monday to Friday 8am - 5pm GMT. 더 많은. HeLa cells were plated at 15,000 cells per well in 100 µL. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Open the catalog to page 1. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Sufficient for 1000 reactions. Introduction. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. 00800 665 32860. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 012 fmol 0. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 314 low lamp level warning. Pick the best speed, sensitivity, and price for your needs. 302 low ref light. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Average 98 stars, based on 1 article reviews. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 1 and above:. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. SoftMax Pro User’s Manual v Contents Figures. 1. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. Analysis. SpectraMax ® iD3 and iD5 Multi-Mode . 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The chamber. The chamber. g. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. The calculated LOQ values were 0. Overview of Western Blot Cartridge. Download SoftMax® Pro 7. Specifically, we present neurite. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. Curves were forced through x,y = 0. If no sound is heard during initialization, contact technical support. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. 7 Figures from 1126 Publications. SpectraMax Microplate Readers. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. china@moldev. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. *On the SpectraMax i3x reader only. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 0. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. Prepare your i3/i3x instrument for shipment. 58% for the detection module and 2. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. Nucleic Acid and Protein Quantitation. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 0. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 99 to $1999. Winnersh Triangle. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Price from $9. D 1 1. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Danaher Inc spectramax i3x. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Promega. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. 3. 3: The cuvette chamber. Download PDF. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. 과 같은 Parameter를 검사하여 평가할 수 있습니다. 6% for the on-board optics (monochromators). Description 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). 100 fmol 1536-well (8 µL) 0. Spectranet customer portal. 01 0. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. MC38 in vivo tumor model in hPD-1. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Wait 20 seconds. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Filterless readers provide consistent results with steady temperature regulation. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. . Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. pathways and protein activation and expression in one system. Explore a wealth of applications with the future-ready SpectraMax i3x. HTRF IP standard curve. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. 2 Parallel Line Analysis Conc log [ng/ML] RLU. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 0´ 104 1. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. View the Molecular Devices website. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Find regional support. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. 306 plate air cal. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Find regional support. 98 / 100 stars. . The calculated LOQ values were 0. Figure 3. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. Explore PDF for more details. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. 0. Use the 3. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 001 0. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Eliminate time-dependent substrate addition steps. ü; ü. 99 to $1999. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. iD5. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. . Introduction. The corrected absorbance is within 5% of the. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. Description. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Controls containing all reaction. Fractional occupancy Corrected BRET ratio NanoBRET. *On the SpectraMax i3x reader only. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 58% for the detection module and 2. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. • Removing the transport locks from the PMT shutters and drawers. ü; SpectraMax® Mini reader support. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. NORTH AMERICA. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. 0001 0. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. Downloads. Example: [Drawer]. . Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. We would like to show you a description here but the site won’t allow us. . Amplitude : 1. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Reporter Assay. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). Quantiferon-TB Gold in Tube Kit. ü; ü. each new assay. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Restart the system. Both readers feature an 8-channel read head for fast reads and. Image analysis provides accurate data. Note: Additional readers with similar performance are listed. Facilities. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Additionally, user-installable options allow the SpectraMax i3 System to. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. . techsupport. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. 0 mm hex key. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. 5 decades, with sensitive detection of cells. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Sensitive TRF detection of two simple, mix-and-measure assay workflows. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 303 unable to cal dark current. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. As. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Molecular Devices. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. . . Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. We would like to show you a description here but the site won’t allow us. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. com. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Published Dec 16, 2020 | Updated Oct 10, 2023. Results were plotted as ratio of green/red vs. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® i3x reader allows you to. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. SpectraMax Microplate Readers. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The SpectraMax i2X is used for cell migration, cell. Simplified workflow: Add cellular imaging assays in a small footprint. Our absorbance plate readers feature our PathCheck Sensor. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Example: [Setup]. i3x. FlexStation® 3 Multi-Mode Microplate Reader. . Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). SpectraMax® ABS and ABS Plus reader support; ü. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. Spectramax I3x, supplied by Danaher Inc, used in various techniques. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SoftMax Pro Software provides integrated instrument control. See SpectraMax® Microplate Reader User Manual Download Page. Contact Us Phone: +1. . Average 98 stars, based on 1 article reviews. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Fluorescent Polarization: Solid black plates, as above. )wideand65. Multimode Readers Comparison Chart. . i3. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. The treatments were. The plate was covered and incubated for one hour at room temperature. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. SpectraMax M5. 2. 2 x 10-14. . Molecular Interactions. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. The information is below the horizontal line. . Wokingham, Berkshire RG41 5TS United Kingdom. SpectraMax Quant dsDNA Assay Kits. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Assay setup for a 384-well low-volume plate. D 1 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Italic and boldface type are used for emphasis. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Download PDF. Capture Flash Assays with Ease. 303 unable to cal dark current. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. Spectramax I3x, supplied by Danaher Inc, used in various techniques. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. 蛍光 発光 ELISA. com. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . +44-118-944-8000. com Check our website for a current listing of worldwide distributors. We would like to show you a description here but the site won’t allow us. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. to View Figures. Get a front row view of your cells. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Thetypeof luciferase(Renilla. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. Figure 4. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. . Get a front row view of your cells. . Not compatible with Home Edition. . The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. .